If you haven’t already heard, Aunt Becky has been traveling the world (seriously, if you have no idea what I’m talking about, click on that link and check out where else she’s been). She was kind enough to make a stop in NYC.
You know how she always talks about being a scientist? And she’s all scientific and shit? Well, I thought I give her a look into the life of a graduate student.
Disclaimer – No graduate students or bacteria were harmed in the making of this story. Nor was Aunt Becky harmed. Please be warned to not try any of this at home unless under the supervision of a Mad Scientist. They can be found in many college centers mumbling to themselves about some solution or other and usually have crazy hair. I must attribute a lot of pictures and use of her body (it’ll make sense soon) to my good friend Z.
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We start off by waking up really early. Well, I usually wake up early and since Aunt Becky was having some trouble sleeping (quite a few trucks drive by at night around here), she woke me up even earlier than usual. Not to worry, I’m a morning person.

GOOD MORNING!
First off, was proper attire. A lab coat is required at all times, as well as closed-toe shoes.

Closed toe-shoes required
(Apparently exposed legs are fine – at least when we are just working with a little bacteria.)
So we got Aunt Becky all set up, we even got her some protection goggles. (See, this is what I mean about using Z’s body. You pervert, what did you think I meant?)

Aunt Becky in Full Lab Attire
And then we were all ready to go. Well, after I take a spin on my super cool stool.

round and round we go
After being a little woozy we got down to business. I wanted to show Aunt Becky how we grow up bacteria in the lab. We wrote out the protocol so we could easily follow what we were doing (always good lab technique).

the protocol
We then took out the plates for the first part of our experiment. We were going to compare the amount of bacteria we each had on our hands. Aunt Becky helped me label the plates.

Labeling Plates
Then we each put our hand (or card) on the plate and let those grow overnight at 37°C (we have an oven in the back).

Aunt Becky in the plate

Inna's fingers in plate
In the mean time, I also wanted to show Aunt Becky how to grow up some bacteria. The first thing we needed to do was pour plates (like the ones you see above) with food for our wonderful little creatures. We put some Agar in these (kind of like gelatin) so that they solidify and we can grow bacteria directly on the surface of the plate.
We made some media. Watch out! Its very very hot! We autoclave the media to make sure its completely sterile and then wait for it to cool enough for us to handle. Thats what that magnet is doing, stirring until its cooler.

media - bacteria food
The next step was pouring the plates. Aunt Becky was quite helpful

pouring plates

continuing to pour
When that was done, we had to leave them on the bench to solidify. You should note, it is very important to have a flame on at all times to ensure your plates are not contaminated. See that blue flame on the right of the plates?

the plates
Now we had a few hours to kill, I thought it would be a good time to feed Aunt Becky. She was getting kind of restless with all this lab stuff and I wasn’t sure what to do. (The mask is for funny purposes only, no masks are gloves are necessary during these experiments with Aunt Becky.)

what do we do now?
Lemon Poppyseed bread muffins would work right? We also had some Diet Dr. Pepper but I don’t want to advertise on this blog (unless I’m getting paid of course – Dr. Pepper, call me!).

lemon poppy seed muffins
Once we had our fill of muffins, it was time to go back to lab and check on the plates. We still had to streak out some bacteria and let those grow overnight as well. To do this, I took a plate that already had bacteria,

those little whitish blobs are colonies of bacteria
picked some up and streaked it onto the Aunt Becky plate with some very colorful toothpicks. They really make all the difference. Not to the bacteria, but to my sanity, all scientists should have colorful toothpicks.

we pick, we streak, we streak again
We then put the Aunt Becky plate in the incubator at 37°C and left only to return the next morning.
At this point (I don’t have any pictures) I don’t really remember what happened next. All I know is that I woke up later that night with a pounding headache and a weird aftertaste in my mouth and a note from Z. She has great handwriting but I had drooled all over it, so you may have to go ask her what it said and what exactly happened to me! But wait, finish reading this story first because you know, we actually grew up bacteria.
The next morning we returned bright an early (Aunt Becky still couldn’t sleep and woke me up). She looked a little battered, but I wasn’t one to question things since we had work to do!
The first plate we looked at was the one of bacteria Aunt Becky helped me streak out. See how nice the lines look? They grew right over the card in the nice lines the toothpicks made.

Aunt Becky streaks out bacteria beautifully!
Then we had to compare who had more bacteria on their hands. Turns out that my hands were much much dirtier than Aunt Becky’s hands. (I must admit I purposefully didn’t wash my hands before we began that experiment.)

Only a single tiny colony on the bottom (see that white spot?)

all the bacteria on my fingers grew very nicely on the plate (you can compare where I laid my fingers down is exactly where bacteria grew)
So that was the end of the experiment. Now you know, wash your hands because we have lots and lots of bacteria. And for those scientists out there, I highly encourage hand washing before starting any experiment. You wouldn’t want any results skewed by your contaminating specimen.
I think Aunt Becky was a bit tired of it all (she now realizes how much work grad school is and how exhausted us grad students get sometimes – I also don’t know what Z did to her because Aunt Becky confessed she’d been up all night because she had been sequestered from my grasp.). Anyways, we have weapons for just the thing at my house, courtesy of McGee#1.



But I convinced her to stay away from the weapons and drink some vodka instead.

mmmm Vodka
And that was the end of our weekend as a MAD SCIENTIST!
Thank you Aunt Becky for stopping by, I had a lovely time, I hope you did as well. Maybe next time Tanna will teach us some cool exploding chemistry tricks.
~ Inna